Coding

Part:BBa_K4825047

Designed by: Ni Weizhao   Group: iGEM23_GreatBay-SCIE   (2023-10-11)


CDC9-LEU1

This compostie part contains the part CDC9 BBa_K4825012 and LEU1 BBa_K4825009. CDC9 encodes DNA ligase (ATP) CDC9 which is located in the mitochondria and nucleus in S. cerevisiae. LEU1 encodes 3-isopropylmalate dehydratase, which is involved in leucine biosynthesis pathway to catalyze the conversion of alpha-isopropylmalate to beta-isopropylmalate.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1163
    Illegal EcoRI site found at 1627
    Illegal EcoRI site found at 2917
    Illegal EcoRI site found at 3706
    Illegal XbaI site found at 1404
    Illegal PstI site found at 2093
    Illegal PstI site found at 2891
    Illegal PstI site found at 3386
    Illegal PstI site found at 3671
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1163
    Illegal EcoRI site found at 1627
    Illegal EcoRI site found at 2917
    Illegal EcoRI site found at 3706
    Illegal PstI site found at 2093
    Illegal PstI site found at 2891
    Illegal PstI site found at 3386
    Illegal PstI site found at 3671
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1163
    Illegal EcoRI site found at 1627
    Illegal EcoRI site found at 2917
    Illegal EcoRI site found at 3706
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1163
    Illegal EcoRI site found at 1627
    Illegal EcoRI site found at 2917
    Illegal EcoRI site found at 3706
    Illegal XbaI site found at 1404
    Illegal PstI site found at 2093
    Illegal PstI site found at 2891
    Illegal PstI site found at 3386
    Illegal PstI site found at 3671
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1163
    Illegal EcoRI site found at 1627
    Illegal EcoRI site found at 2917
    Illegal EcoRI site found at 3706
    Illegal XbaI site found at 1404
    Illegal PstI site found at 2093
    Illegal PstI site found at 2891
    Illegal PstI site found at 3386
    Illegal PstI site found at 3671
    Illegal NgoMIV site found at 2434
    Illegal AgeI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3896


[edit]
Categories
Parameters
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